Wheat Triticum aestivum L starch synthase II which is also known as starch granule protein 1 SGP1 plays a major role in endosperm starch synthesis The three SGP1 proteins SGPA1 B1 and D1 are produced by three homoeologous SSII genes wSSIIA B and D Lines carrying null alleles for each SGP1 protein have previously been identified In this report the mutations occurring in
Mutations in wheat starch synthase II genes and PCR ResearchGate
Genetic elimination of a starch granule protein SGP1 of wheat
Wheat has three isoforms of SGP1 SGPA1 B1 and D1 The gene coding for SGPA1 SgpA1 is located on chromosome arm 7AS SgpB1is on 7BS and SgpD1is on 7DS Denyer et al 1995 Yamamori and Endo 1996 Using SDSPAGE Yamamori and Endo 1996 found that a few wheat cultivars lacked either SGPA1 B1 or D1 but no wheats lacked more than
The three SGP1 proteins SGPA1 B1 and D1 are produced by three homoeologous SSII genes wSSIIA B and D Lines carrying null alleles for each SGP1 protein have previously been identified
Genetic elimination of a starch granule protein SGP1 of wheat
Mutations in wheat starch synthase II genes and PCRbased PubMed
The SGPA1 nulls were crossed with the durum variety Mountrail and F 5 derived SGPA1 null progeny lines were treated with EMS From each EMS population one SGPB1 null mutation was recovered with each being a missense mutation Each of the SGP1 nulls was found to have large increases in amylose content and reduced binding of SGP2 and
Sgp A1
Two different electrophoretic profiles were identified SGPA1 proteins of T urartu accessions had a SDSPAGE mobility similar to those of tetraploid and hexaploid wheat species conversely SGPA1 proteins of T monococcum ssp monococcum and ssp boeoticum accessions showed a different electrophoretic mobility The entire coding region of
Molecular characterisation of two novel starch granule proteins 1 in
Creation of a highamylose durum wheat through mutagenesis of starch synthase II SSIIa
The Localization and Expression of the Class II Starch Synthases of
A starch granule protein SGP1 is a starch synthase bound to starch granules in wheat endosperm A wheat lacking SGP1 was produced by crossing three variants each deficient in one of three SGP1 classes namely SGPA1 B1 or D1 This deficient wheat SGP1 null wheat showed some alterations in endosperm starch meaning that SGP1 is involved in starch synthesis Electrophoretic
The three SGP1 proteins SGPA1 B1 and D1 are produced by three homoeologous SSII genes wSSIIA B and D Lines carrying null alleles for each SGP1 protein have previously been identified In this report the mutations occurring in each wSSII gene were characterized and PCRbased DNA markers capable of detecting the mutations were
Sgp A1
PDF Genetic elimination of a starch granule protein SGP1 of wheat
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A wheat lacking SGP1 was produced by crossing three variants each deficient in one of three SGP1 classes namely SGPA1 B1 or D1 This deficient wheat SGP1 null wheat showed some
Given the similarity in the expression of the SGPA1 AGPB1 and SGPD1 proteins in the granule fraction Rahman et al 1995 it is reasonable to assume that SGPA1 and SGPD1 are also found in the soluble fraction during early endosperm development however the very tight specificity of the monoclonal antibody available precludes our
Mutations in wheat starch synthase II genes and PCRbased Springer